![]() According to the latest criteria of International Committee on Taxonomy of Viruses, CoVs are classified into four genera, including Alphacoronavirus, Betacoronavirus, Deltacoronavirus, and Gammacoronavirus. ![]() We propose that this method be used to complement next generation sequencing technology for large-scale screening studies.Ĭoronaviruses (CoVs) are large, enveloped, positive-sense RNA viruses that can cause respiratory, enteric, hepatic, and neurological diseases in a range of animals, including humans ( Lai et al., 2007). ![]() The mCoV-MS method is a sensitive assay that is relatively simple to carry out. The validation test showed mCoV-MS method can detect potentially pathogenic CoVs in Alphacoronavirus and Betacoronavirus and provide convincingly phylogenetic evidences about unknown CoVs. The results showed good concordance with the results of metagenomic analysis or PCR-sequencing. To confirm the usefulness of the mCoV-MS method we screened 384 pharyngeal and/or anal swab samples collected from bats/rodents, and 131 nasal and throat swabs from human patients. All tested primers and probes for the mCoV-MS method were effective, with no cross-reactivity observed with other common respiratory viruses. We developed a 17-plex analysis to detect six HCoVs in Panel A and another 17-plex analysis to detect Alphacoronavirus and Betacoronavirus in Panel B. The method was performed using a MassARRAY matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. Our work aims to develop a sensitive method (mCoV-MS) which can not only identify known HCoVs accurately, but also have the ability to provide clues for the emerging HCoVs. The discovery of severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV shows that HCoVs pose a significant threat to human health. There are four human coronaviruses (HCoVs), distributed worldwide, that are associated with a range of respiratory symptoms. Ministry of Health Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.Leshan Xiu †, Chi Zhang †, Zhiqiang Wu and Junping Peng *
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